Title: Generation and functional characterization of a conditional Pumilio2 null allele
Authors: Kaibo Lin, Shikun Zhang, Jieli Chen, Ding Yang, Mengyi Zhu, Eugene Yujun Xu
Institutions: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
Highly conserved RNA binding protein PUF (Pumilio/FBF) family, is present throughout eukaryotes from yeast to mammals, with critical roles in development, fertility and nervous system. However, function of mammalian PUF family members remained underexplored. Our previous study reported that a gene-trap mutation of Pum2 results in smaller testis but does not impact fertility and viability. Although the gene-trap mutation disrupted the key functional domain of PUM protein–PUM-HD (Pumilio homology domain) but still produced a chimeric Pum2-?-geo protein containing part of PUM2 protein, raising a question if such a chimeric protein might provide any residual function or contribute to the reproductive phenotype. Here we report the generation of a conditional PUM2 allele, when knockedout, producing no residual PUM2 protein and hence a complete loss-of-function allele. We also uncovered small but significant reduction of male fertility and viability in the mutants, suggesting requirements of PUM2 in male fertility and viability.
Keywords: RNA-binding protein, Pumilio2, PUF, fertility, viability
J Biomed Res published on 31 December 2017, doi: https://doi.org/10.7555/JBR.32.20170117