Title: Profiles of metabolic gene expression in the white adipose tissue, liver and hypothalamus in leptin knockout rats (LepΔI14/ΔI14)


Authors: Leijian Guan1, Kaixuan Xu1,2, Shuyang Xu3, Ningning Li1, Xinru Wang1, Yankai Xia1, Di Wu1


Institutions: 1State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China; 2Xuzhou Central Hospital, The Affiliated Xuzhou Hospital of Medical College of Southeast University, Xuzhou, Jiangsu 221009, China; 3School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.


Abstract: Leptin deficiency is principally linked to metabolic disorders. Leptin knockout (LepΔI14/ΔI14) Sprague Dawley rats created by CRISPR/Cas9 is a new model to study metabolic disorders. We used a whole rat genome oligonucleotide microarray to obtain tissue-specific gene expression profiles of the white adipose tissue, liver and hypothalamus in LepΔI14/ΔI14 and wild-type (WT) rats. We found 1,651 differentially expressed (enriched) genes in white adipose tissue, 916 in the liver, and 306 in the hypothalamus in the LepΔI14/ΔI14 rats compared to WT. Gene ontology category and KEGG pathway analysis of the relationships among differentially expressed genes showed that these genes were represented in a variety of functional categories, including fatty acid metabolism, molecular transducers and cellular processes. The reliability of the data obtained from microarray was verified by quantitative real-time PCR on 14 representative genes. These data will contribute to a greater understanding of different metabolic disorders, such as obesity and diabetes.


Keywords: LepΔI14/ΔI14, microarray analysis, white adipose, liver, hypothalamus


Full Text: JBR-2017-0021.pdfJBR-2017-0021 supplementary.pdf


J Biomed Res published on 28 April, 2017, doi:10.7555/JBR.31.20170021