Title: Proteomic analysis of the dorsal spinal cord in the mouse model of spared nerve injury-induced neuropathic pain

 

Authors: Eun-sung Park1, Jung-mo Ahn2, Sang-min Jeon3, Hee-jung Cho3, Ki-myung Chung4, Je-yoel Cho5, Dong-ho Youn1

 

Institutions: 1Department of Oral Physiology, School of Dentistry, Kyungpook National University, Jung-gu, Daegu 41940, R. O. Korea; 2Biocenter, Incheon Technopark, Yeonsu-gu, Incheon 406-840, R. O. Korea; 3Department of Anatomy, School of Medicine, Kyungpook National University, Dong-in Dong, Daegu 700-422, R. O. Korea; 4Department of Physiology and Neuroscience, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung,Gangwon-do 210-702, R. O. Korea; 5Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul 151-742, R. O. Korea.

 

Abstract: Peripheral nerve injury often causes neuropathic pain and is associated with changes in the expression of numerous proteins in the dorsal horn of the spinal cord. To date, proteomic analysis method has been used to simultaneously analyze hundreds or thousands of proteins differentially expressed in the dorsal horn of the spinal cord in rats or dorsal root ganglion of rats with certain type of peripheral nerve injury. However, a proteomic study using a mouse model of neuropathic pain could be attempted because of abundant protein database and the availability of transgenic mice. In this study, whole proteins were extracted from the ipsilateral dorsal half of the 4th6th lumbar spinal cord in a mouse model of spared nerve injury (SNI)-induced neuropathic pain. In-gel digests of the proteins size-separated on a polyacrylamide gel were subjected to reverse-phase liquid-chromatography coupled with electrospray ionization ion trap tandem mass spectrometry (MS/MS). After identifying proteins, the data were analyzed with subtractive proteomics using ProtAn, an in-house analytic program. Consequently, 15 downregulated and 35 upregulated proteins were identified in SNI mice. The identified proteins may contribute to the maintenance of neuropathic pain, and may provide new or valuable information in the discovery of new therapeutic targets for neuropathic pain.

 

Keywords: proteomics, spinal dorsal horn, neuropathic pain, spared nerve injury, mouse

 

Full Text: JBR-2016-0122.pdf

 

J Biomed Res  published on 22 January, 2017, doi:10.7555/JBR.31.20160122