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Leijian Guan,Kaixuan Xu,Shuyang Xu,Ningning Li,Xinru Wang,Yankai Xia,Di Wu.Journal of Biomedical Research,2017,31(6):528-540
Profiles of metabolic gene expression in the white adiposetissue, liver and hypothalamus in leptin knockout (LepI14/I14 )rats
Received:March 01, 2017  Revised:March 18, 2017
DOI10.7555/JBR.31.20170021
KeywordsLepI14/I14, microarray analysis, white adipose, liver, hypothalamus
Grant ProgramWe gratefully acknowledge the technical assistance of Ling Song. This work was supported by grants from the National Natural Science Foundation of China (No. 81470150).
AuthorInstitution
Leijian Guan State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing , China
Kaixuan Xu State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing , China;Xuzhou Central Hospital, The Affiliated Xuzhou Hospital of Medical College of Southeast University, Xuzhou, Jiangsu , China
Shuyang Xu School of Life Sciences and Technology, Tongji University, Shanghai , China
Ningning Li State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing , China
Xinru Wang State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing , China
Yankai Xia State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing , China
Di Wu State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing , China
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Abstract
      Leptin deficiency is principally linked to metabolic disorders. Leptin knockout (LepI14/I14) Sprague Dawley rats created by CRISPR/Cas9 is a new model to study metabolic disorders. We used a whole rat genome oligonucleotide microarray to obtain tissue-specific gene expression profiles of the white adipose tissue, liver and hypothalamus in LepI14/I14 and wild-type (WT) rats. We found 1,651 differentially expressed (enriched) genes in white adipose tissue, 916 in the liver, and 306 in the hypothalamus in the LepI14/I14 rats compared to WT. Gene ontology category and KEGG pathway analysis of the relationships among differentially expressed genes showed that these genes were represented in a variety of functional categories, including fatty acid metabolism, molecular transducers and cellular processes. The reliability of the data obtained from microarray was verified by quantitative real-time PCR on 14 representative genes. These data will contribute to a greater understanding of different metabolic disorders, such as obesity and diabetes.
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