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Li Xin,Tian Han,Jiao Tang,Xiaoyu Wang,Hao Zhang,Hongyan Dong,Kaijin Guo,Zhongming Zhang.Journal of Biomedical Research,2017,31(6):512-520
Pigment epithelium derived factor (PEDF) prevents methylmethacrylate monomer-induced cytotoxicity in H9c2 cells
Received:June 25, 2017  Revised:September 25, 2017
DOI10.7555/JBR.31.20170068
Keywordspigment epithelium-derived factor, oxidative stress, bone cement, methyl methacrylate
Grant ProgramThis work was supported by the National Natural Science Foundation of China (81270173), Jiangsu government grant to study abroad (JS-2013-246) and Xuzhou Science and Technology Projects (XZZD- 1329)
                       
AuthorInstitution
Li Xin Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu , China;Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu , China
Tian Han Department of Orthopaedics, Xuzhou First People's Hospital, Xuzhou, Jiangsu , China
Jiao Tang Department of Neurology, Yancheng City No.1 People's Hospital, Yancheng, Jiangsu , China
Xiaoyu Wang Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu , China
Hao Zhang Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu , China
Hongyan Dong Research Facility Center for Morphology, Xuzhou Medical University, Xuzhou, Jiangsu , China
Kaijin Guo Department of Orthopaedics, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu , China
Zhongming Zhang Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu , China;Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu , China
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Abstract
      Acrylic bone cements are currently the most frequently and extensively used materials in orthopedic implant treatment. However, adverse effects have been described of acrylic bone cement on the cardiovascular system. In the present study, we examined the cytotoxicity of bone cement ingredient methyl methacrylate (MMA) to cardiomyocytes and the potential detoxifying effect of pigment epithelium-derived factor (PEDF) in H9c2 cells. We found that high concentration of MMA ( > 120 mmol/L) led to necrotic cell death in H9c2 cells. However, MMA at low concentrations (30-90 mmol/L) caused apoptosis. Pretreatment of PEDF prevented MMA-induced cytotoxicity. In addition, PEDF enhanced total superoxide dismutase activities, and decreased MMA-induced production of malonaldehyde. Furthermore, MMA-induced downregulation of Akt activity was suppressed by PEDF. PEDF also increased the levels of peroxisome proliferator activated receptor gamma (PPARg) and lysophosphatidic acids (LPA) through PEDF receptor. These results indicated that PEDF inhibited MMA-induced cytotoxicity through attenuating oxidative stress, activating the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and/or PEDF receptorLPA-PPARg pathways in H9c2 cells. PEDF may be explored as a candidate therapeutic agent for alleviating bone cement implantation syndrome during orthopedic surgery.
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