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Eun-sung Park,Jung-mo Ahn,Sang-min Jeon,Hee-jung Cho,Ki-myung Chung,Je-yoel Cho,Dong-ho Youn.Journal of Biomedical Research,2017,31(6):494-502
Proteomic analysis of the dorsal spinal cord in the mouse modelof spared nerve injury-induced neuropathic pain
Received:September 26, 2016  Revised:December 10, 2016
DOI10.7555/JBR.31.20160122
Keywordsproteomics, spinal dorsal horn, neuropathic pain, spared nerve injury, mouse
Grant ProgramThis research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2015R1D1A1A01059432).
                    
AuthorInstitution
Eun-sung Park Department of Oral Physiology, School of Dentistry, Kyungpook National University, Jung-gu, Daegu 41940, R. O. Korea
Jung-mo Ahn Biocenter, Incheon Technopark, Yeonsu-gu, Incheon 406-840, R. O. Korea
Sang-min Jeon Department of Anatomy, School of Medicine, Kyungpook National University, Dong-in Dong, Daegu 700-422, R. O. Korea
Hee-jung Cho Department of Anatomy, School of Medicine, Kyungpook National University, Dong-in Dong, Daegu 700-422, R. O. Korea
Ki-myung Chung Department of Physiology and Neuroscience, College of Dentistry and Research Institute of Oral Science, GangneungWonju National University, Gangneung,Gangwon-do 210-702, R. O. Korea
Je-yoel Cho Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul 151-742, R. O. Korea
Dong-ho Youn Department of Oral Physiology, School of Dentistry, Kyungpook National University, Jung-gu, Daegu 41940, R. O. Korea
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Abstract
      Peripheral nerve injury often causes neuropathic pain and is associated with changes in the expression of numerous proteins in the dorsal horn of the spinal cord. To date, proteomic analysis method has been used to simultaneously analyze hundreds or thousands of proteins differentially expressed in the dorsal horn of the spinal cord in rats or dorsal root ganglion of rats with certain type of peripheral nerve injury. However, a proteomic study using a mouse model of neuropathic pain could be attempted because of abundant protein database and the availability of transgenic mice. In this study, whole proteins were extracted from the ipsilateral dorsal half of the 4thC6th lumbar spinal cord in a mouse model of spared nerve injury (SNI)-induced neuropathic pain. In-gel digests of the proteins size-separated on a polyacrylamide gel were subjected to reverse-phase liquid-chromatography coupled with electrospray ionization ion trap tandem mass spectrometry (MS/MS). After identifying proteins, the data were analyzed with subtractive proteomics using ProtAn, an in-house analytic program. Consequently, 15 downregulated and 35 upregulated proteins were identified in SNI mice. The identified proteins may contribute to the maintenance of neuropathic pain, and may provide new or valuable information in the discovery of new therapeutic targets for neuropathic pain.
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