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Houcheng Liang,Jingdong Zhang,Pifu Luo,Hongna Zhu,Ying Qiao,Anle Su,Ting Zhang.Journal of Biomedical Research,2017,31(3):256-263
Electromyography and Fos immunostaining study establish a possible functional link between trigeminal proprioception and the oculomotor system in rats
Received:September 27, 2016  Revised:December 13, 2016
DOI10.7555/JBR.31.20160127
KeywordsMarcus Gunn syndrome, down-stretching lower jaw, levator and superior rectus, electromyography, interstitial nucleus of Cajal and Darkschewitsch nucleus, Fos expression
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Houcheng Liang Xi'an BRIGHT Eye Hospital, Xi'an, Shaanxi , China;Shaanxi Provincial Eye Research Institute and Xi'an Eye Hospital Xi'an, Shaanxi , China;Department of Ophthalmology, 2nd Affiliated Hospital of Xi'an Jiao-Tong University, Xi'an, Shaanxi , China
Jingdong Zhang Xi'an BRIGHT Eye Hospital, Xi'an, Shaanxi , China
Pifu Luo Department of Pathology, KingMed Diagnostics, International Bioisland, Guangzhou, Guangdong , China
Hongna Zhu Shaanxi Provincial Eye Research Institute and Xi'an Eye Hospital Xi'an, Shaanxi , China
Ying Qiao Shaanxi Provincial Eye Research Institute and Xi'an Eye Hospital Xi'an, Shaanxi , China
Anle Su Shaanxi Provincial Eye Research Institute and Xi'an Eye Hospital Xi'an, Shaanxi , China
Ting Zhang Shaanxi Provincial Eye Research Institute and Xi'an Eye Hospital Xi'an, Shaanxi , China
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Abstract
      The objective of this study was to explore whether there was a functional link between trigeminal proprioception and the oculomotor system mediated through jaw muscle afferents. Electromyography (EMG) was undertaken of the levator palpebrae (LP) and superior rectus (SR), and Fos expression was detected in the brainstem following consecutive down-stretching of the lower jaw at 2-4 Hz in rats. Retrograde tracing was undertaken of the interstitial nucleus of Cajal and Darkschewitsch nucleus (INC/DN) pre-oculomotor neurons. EMG-like responses were recorded from the LP/SR during down-stretching of the lower jaw at 2-4 Hz in 3 out of 11 rats. Fos expression was induced by consecutive down-stretching of the lower jaw at 2-4 Hz for 20-30 seconds. Interestingly, Fos expression was distributed mainly in the bilateral INC/DN area. We also examined Fos-like immunoreactivity in central mesencephalic and paramedian pontine reticular formation that harbors premotor neurons controlling horizontal eye movement, but no Fos-like staining was observed therein. By injection of retrograde tracers into the oculomotor nucleus combined with Fos immunostaining, double labeled pre-oculomotor neurons were visualized to distribute in the INC/DN. In conclusions, there may exist a trigeminal proprioceptive C oculomotor system neural circuit through jaw muscle afferents in rats. Judging from Fos distribution pattern, this pathway might be related to vertical and torsional eye movements.
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