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Wei Li,Wu Di,Lijuan Hua,Bingrong Zhou,Ze Guo,Dan Luo.Journal of Biomedical Research,2011,25(2):135-140
UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells
  
DOI10.1016/S1674-8301(11)60017-1
KeywordsMicroRNA-141, PTEN, UVB, apoptosis, HaCaT cells
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Wei Li Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu , China
Wu Di Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu , China
Lijuan Hua Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu , China
Bingrong Zhou Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu , China
Ze Guo Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu , China
Dan Luo Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu , China
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Abstract
      MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucle-otide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB ir-radiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.
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