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Joshua Muli Mutiso,John Chege Macharia,Evans Taracha,Michael Muita Gicheru.Journal of Biomedical Research,2012,26(1):8-16
Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops)
Received:July 23, 2011  
DOI£º10.1016/S1674-8301(12)60002-5
Keywords£ºvisceral leishmaniasis, Leishmania donovani, vervet monkey, sonicated antigen, adjuvants
Grant Program£º
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AuthorInstitution
Joshua Muli Mutiso Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya/Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya
John Chege Macharia Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya
Evans Taracha Department of Tropical and Infectious Diseases, Institute of Primate Research, Karen, Nairobi 24481-00502, Kenya
Michael Muita Gicheru Department of Zoological Sciences, Kenyatta University, Nairobi 43844-00100, Kenya
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Abstract £º
      In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicity and efficacy of Leishmania donovani (L. donovani) sonicated antigen delivered with alum-BCG (AlBCG), MISA or monophosphoryl lipid A (MPLA) in vervet monkeys following intradermal inoculums. Vaccinated and control animals were challenged with virulent L. donovani parasites and the parasitic burden was determined. Only animals vaccinated with alum-BCG adversely reacted to the inoculum by produc-ing ulcerative erythematous skin indurations. Non-parametric ANOVA followed by a post test showed signifi-cantly higher IgG antibodies, and revealed the presence of lymphoproliferative and interferon gamma responses in both AlBCG+Ag and MISA+Ag as compared to the MPLA+Ag or other groups (P < 0.001). We conclude that L. donovani sonicated antigen containing MISA is safe and is associated with protective immune response against Leishmania donovani infection in the vervet monkey model.
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